| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-96990 | |||
| FITC | NBP1-96856 | |||
| Rhodamine | NBP1-96857 | |||
| Texas Red | NBP1-96985 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB120-7055 | |||
| FITC | NB120-7050-1000ug | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP3-11660 | |||
| DyLight 680 | NBP3-11664 | |||
| DyLight 800 | NBP3-11663 | |||
| FITC | NBP3-11662 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-97291 | |||
| FITC | NBP1-97272 | |||
| Rhodamine | NBP1-97271 | |||
| Texas Red | NBP1-97292 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Purified | NBP1-96772 | |||
![Dot Blot: Transferrin Native Protein [NBP1-97069] - Dot Blot result of Human Transferrin Peroxidase conjugate. Dots are Human Transferrin HRP at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Block for 60 min at RT. Primary Antibody: none. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Chemi filter.](http://images.novusbio.com/fullsize/Transferrin-Native-Protein-Dot-Blot-NBP1-97069-img0001.jpg "Dot Blot: Transferrin Native Protein [NBP1-97069] - Dot Blot result of Human Transferrin Peroxidase conjugate. Dots are Human Transferrin HRP at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Block for 60 min at RT. Primary Antibody: none. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Chemi filter.")
![Western Blot: Streptavidin Full length Protein [NBP2-29370] - Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none.](http://images.novusbio.com/fullsize/Streptavidin-Full-length-Protein-Western-Blot-NBP2-29370-img0002.jpg "Western Blot: Streptavidin Full length Protein [NBP2-29370] - Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none.")
![Western Blot: Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB120-7171] - Western blot of Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [HRP].Lane 1: Rabbit IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [HRP] at 1:1000 for 60 min at RT.Blocking: Fluorescent blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.Other band(s): None.](http://images.novusbio.com/fullsize/Goat-F-ab-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB120-7171-img0006.jpg "Western Blot: Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB120-7171] - Western blot of Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [HRP].Lane 1: Rabbit IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody [HRP] at 1:1000 for 60 min at RT.Blocking: Fluorescent blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.Other band(s): None.")
![Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Western Blot of Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP]. Lane 1: Mouse IgG, non-denatured. Lane 2: 5 uL Opal Pre-stained Ladder [HRP] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 160 kDa non-denatured for Mouse IgG.](http://images.novusbio.com/fullsize/Rat-Pure-Blot-anti-Mouse-IgG-Secondary-Antibody-eB144-HRP-Western-Blot-NBP3-11661-img0002.jpg "Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Western Blot of Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP]. Lane 1: Mouse IgG, non-denatured. Lane 2: 5 uL Opal Pre-stained Ladder [HRP] at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 160 kDa non-denatured for Mouse IgG.")
![Western Blot: Rat Pure-Blot anti-Mouse IgG (H+L) Secondary Antibody (eB144) [HRP] [NBP3-11661] - Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP] (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Rat Pure-Blot anti-Mouse IgG Secondary antibody (eB144) [HRP] detects only native antibody and not denatured heavy and light chains.](http://images.novusbio.com/fullsize/Rat-Pure-Blot-anti-Mouse-IgG-Secondary-Antibody-eB144-HRP-Western-Blot-NBP3-11661-img0001.jpg)
![Sheep anti-Rat IgG (H+L) Secondary Antibody [HRP] [NBP2-22109] - Western Blot analysis using Sheep anti-Rat IgG (H+L) Secondary antibody [HRP] (50ng/lane). Lane M: Molecular Ladder Lane 1: Rat IgG whole molecule Lane 2: Rat IgG F(c) fragment Lane 3: Rat IgG Fab fragment Lane 4: Rat IgM whole molecule Lane 5: Rat Serum Primary antibody: at 1:1000 for 60min at RT. Secondary antibody: Donkey anti-Sheep IgG (Peroxidase) at 1:40,000 in blocking buffer for 30min at RT. Block: Blocking buffer for 30min at RT](http://images.novusbio.com/fullsize/Sheep-anti-Rat-IgG-H+L-Secondary-Antibody-HRP-NBP2-22109-img0001.jpg "Sheep anti-Rat IgG (H+L) Secondary Antibody [HRP] [NBP2-22109] - Western Blot analysis using Sheep anti-Rat IgG (H+L) Secondary antibody [HRP] (50ng/lane). Lane M: Molecular Ladder Lane 1: Rat IgG whole molecule Lane 2: Rat IgG F(c) fragment Lane 3: Rat IgG Fab fragment Lane 4: Rat IgM whole molecule Lane 5: Rat Serum Primary antibody: at 1:1000 for 60min at RT. Secondary antibody: Donkey anti-Sheep IgG (Peroxidase) at 1:40,000 in blocking buffer for 30min at RT. Block: Blocking buffer for 30min at RT")
![Western Blot: Myeloperoxidase/MPO Antibody [NBP1-77779] - Analysis of Rabbit anti-Myeloperoxidase antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: MCF-7 Lysate. Lane 4: Jurkat Lysate. Lane 5: A549 Lysate. Lane 6: HL-60 Lysate. Lane 7: Raji Lsyate. Lane 8: NIH/3T3 Lysate. Primary antibody: Myeloperoxidase antibody.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-Western-Blot-NBP1-77779-img0001.jpg "Western Blot: Myeloperoxidase/MPO Antibody [NBP1-77779] - Analysis of Rabbit anti-Myeloperoxidase antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: MCF-7 Lysate. Lane 4: Jurkat Lysate. Lane 5: A549 Lysate. Lane 6: HL-60 Lysate. Lane 7: Raji Lsyate. Lane 8: NIH/3T3 Lysate. Primary antibody: Myeloperoxidase antibody.")
![SDS-Page: Rabbit IgG Fc Isotype Control [HRP] [NBP1-97241] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment Peroxidase Conjugated. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.](http://images.novusbio.com/fullsize2/Rabbit-IgG-Fc-Isotype-Control-[HRP]-SDS-Page-NBP1-97241-img0001.jpg "SDS-Page: Rabbit IgG Fc Isotype Control [HRP] [NBP1-97241] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment Peroxidase Conjugated. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.")